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STEMCELL Technologies Inc stemspantm sfem ii medium
Stemspantm Sfem Ii Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemspantm+sfem+medium/stemspantm+sfem+ii+medium/pmc12278485-32-4-8
Average 90 stars, based on 1 article reviews
stemspantm sfem ii medium - by Bioz Stars, 2026-09
90/100 stars

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Cell Culture:

Article Title: BMP signaling promotes zebrafish heart regeneration via alleviation of replication stress
Article Snippet: CD34+ HSPCs were enriched using the CD34 Micro Bead Kit (Miltenyi Biotec) according to the manufacturer ́s instructions. .. HSPCs were cultured for 24 h at 37 °C, 5% CO 2 and ambient O 2 in serum free StemSpanTM SFEM medium (STEMCELL Technologies) supplemented with 1% Penicillin/Streptomycin (Gibco by Thermo Fisher Scientific) and 1% StemSpanTM CC100 (STEMCELL Technologies,) before treatment with either 50 ng/ml BMP2 or BMP4 (R&D Systems #355-BM-010 and #314-BP-010) solved in 0.1% BSA (Sigma-Aldrich by Merck) for 48 h. ..

Article Title: BMP signaling promotes heart regeneration via alleviation of replication stress
Article Snippet: CD34+ HSPCs were enriched by using the CD34 Micro Bead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufactureŕs description. .. HSPCs were cultured for 24 h at 37°C, 5% CO 2 and ambient O 2 in serum free StemSpanTM SFEM medium (STEMCELL Technologies, Cologne, Germany) supplemented with 1% Penicillin/Streptomycin (Gibco by Thermo Fisher Scientific, Waltham, Massachusetts, USA) and 1% StemSpanTM CC100 (STEMCELL Technologies, Cologne, Germany) before treatment with either 50 ng/ml BMP2 or BMP4 (#355-BM-010 and #314-BP-010, R&D Systems, Minnesota, USA) solved in 0.1% BSA (Sigma-Aldrich by Merck, Darmstadt, Germany) for 48 h. U2OS cells were cultivated in DMEM high glucose (Gibco by Thermo Fisher Scientific, Waltham, Massachusetts, USA) supplemented with 10% FBS (Biochrom by Merck, Darmstadt, Germany) at 37°C, 5% CO 2 and ambient O 2 . .. For BMP stimulation cells were transferred into serum-free medium X-VIVO 10 (Lonza, Walkersville, Maryland, USA).

Article Title: In vivo monitoring of leukemia-niche interactions in a zebrafish xenograft model.
Article Snippet: .. For short-term in vitro culture, cells were cultured in StemSpanTM SFEM Medium (STEMCELL Technologies, Vancouver, Canada). .. For cell proliferation analysis, cells were stained with 5 μM CFSE (Invitrogen™, Waltham, MA, USA) and resuspended in the respective medium for overnight cultivation.

Article Title: Global Transcriptomic and Characteristics Comparisons between Mouse Fetal Liver and Bone Marrow Definitive Erythropoiesis.
Article Snippet: The cells were imaged using a Leica DM2000 inverted microscope (Leica Microsystems, Buffalo Grove, IL, USA). .. Single BFU-E or CFU-E cells were sorted to a 96-well plate and cultured with StemSpanTM SFEM medium (STEMCELL Technologies, Vancouver, BC, Canada) containing 100 ng/mL recombinant murine stem cell factor (rmSCF, STEMCELL Technologies, Vancouver, BC, Canada), 40 ng/mL recombinant murine insulin-like growth factor-1 (rmIGF-1, STEMCELL Technologies, Vancouver, BC, Canada), 2 U/mL recombinant human erythropoietin (rhEPO, Gibco, Billings, MT, USA), 100 U/mL penicillin and 100 μg/mL streptomycin and incubated at 37 ◦C in a humidified atmosphere (relative humidity > 80%) with 5% CO2. .. Sorted BM and FL erythroid progenitors were stained with CellTraceTM Violet dye (Thermo Fisher Scientific, Eugene, OR, USA) according to the instructions from the manufacturer and cultured in the medium as described above.

Article Title: Generation of an induced pluripotent stem cell line (SSMCi001-A) from a dilated cardiomyopathy patient due to mutations in the TTN gene.
Article Snippet: .. PBMCs (1 × 106–5 × 106 cells) were initially cultured in StemSpanTM SFEM medium (STEMCELL Technologies) supplemented with StemSpanTM CC100 cytokine cocktail for 7 days. .. Cellular reprogramming was performed using episomal vectors through electroporation with the AmaxaTM Human CD34+ Cell NucleofectorTM Kit (Lonza) using program T-020.

Article Title:
Article Snippet: Human AML cell lines, such as THP-1 (ATCC), U937 (ATCC) or MV4-11 (ATCC), were cultured in the RPMI-1640 medium containing 10% FBS and subjected to the analysis of cell proliferation changes after P2X1 knockdown with its specific shRNAs (sh-P2X1 #1, #2 or scrambled) or after infected with empty vector (EV), WT or mutant P2X1 (S387A or T389A) plasmids at the indicated time points. .. For analyzing the proliferation abilities of human cord blood CD34+ cells, the indicated numbers of CD34+ cells were cultured in StemSpanTM SFEM medium (Stemcell Technologies) supplemented with 100 ng/ml SCF, 100 ng/ml TPO and 100 ng/ml FLT3-L. ..

Article Title: Establishment of an iPSC line from a NDD patient with a heterozygous mutation in the CTNNB1 gene.
Article Snippet: CTNNB1 encodes beta-catenin, which plays a crucial role in Wnt signaling pathway.. Mutations in CTNNB1 involve in tumor developing, Primary Aldosteronism, Neurodevelopmental disorders (NDDs), etc.. NDDs is a class of disorders that impact brain development and function, manifesting symptom including autism spectrum disorder (ASD), intellectual disability (ID), schizophrenia (SCZ), and epilepsy.

Article Title: Global Transcriptomic and Characteristics Comparisons between Mouse Fetal Liver and Bone Marrow Definitive Erythropoiesis
Article Snippet: The cells were imaged using a Leica DM2000 inverted microscope (Leica Microsystems, Buffalo Grove, IL, USA). .. Single BFU-E or CFU-E cells were sorted to a 96-well plate and cultured with StemSpanTM SFEM medium (STEMCELL Technologies, Vancouver, BC, Canada) containing 100 ng/mL recombinant murine stem cell factor (rmSCF, STEMCELL Technologies, Vancouver, BC, Canada), 40 ng/mL recombinant murine insulin-like growth factor-1 (rmIGF-1, STEMCELL Technologies, Vancouver, BC, Canada), 2 U/mL recombinant human erythropoietin (rhEPO, Gibco, Billings, MT, USA), 100 U/mL penicillin and 100 μg/mL streptomycin and incubated at 37 °C in a humidified atmosphere (relative humidity > 80%) with 5% CO 2 . .. Sorted BM and FL erythroid progenitors were stained with CellTraceTM Violet dye (Thermo Fisher Scientific, Eugene, OR, USA) according to the instructions from the manufacturer and cultured in the medium as described above.

In Vitro:

Article Title: In vivo monitoring of leukemia-niche interactions in a zebrafish xenograft model.
Article Snippet: .. For short-term in vitro culture, cells were cultured in StemSpanTM SFEM Medium (STEMCELL Technologies, Vancouver, Canada). .. For cell proliferation analysis, cells were stained with 5 μM CFSE (Invitrogen™, Waltham, MA, USA) and resuspended in the respective medium for overnight cultivation.

Recombinant:

Article Title: Global Transcriptomic and Characteristics Comparisons between Mouse Fetal Liver and Bone Marrow Definitive Erythropoiesis.
Article Snippet: The cells were imaged using a Leica DM2000 inverted microscope (Leica Microsystems, Buffalo Grove, IL, USA). .. Single BFU-E or CFU-E cells were sorted to a 96-well plate and cultured with StemSpanTM SFEM medium (STEMCELL Technologies, Vancouver, BC, Canada) containing 100 ng/mL recombinant murine stem cell factor (rmSCF, STEMCELL Technologies, Vancouver, BC, Canada), 40 ng/mL recombinant murine insulin-like growth factor-1 (rmIGF-1, STEMCELL Technologies, Vancouver, BC, Canada), 2 U/mL recombinant human erythropoietin (rhEPO, Gibco, Billings, MT, USA), 100 U/mL penicillin and 100 μg/mL streptomycin and incubated at 37 ◦C in a humidified atmosphere (relative humidity > 80%) with 5% CO2. .. Sorted BM and FL erythroid progenitors were stained with CellTraceTM Violet dye (Thermo Fisher Scientific, Eugene, OR, USA) according to the instructions from the manufacturer and cultured in the medium as described above.

Article Title: Global Transcriptomic and Characteristics Comparisons between Mouse Fetal Liver and Bone Marrow Definitive Erythropoiesis
Article Snippet: The cells were imaged using a Leica DM2000 inverted microscope (Leica Microsystems, Buffalo Grove, IL, USA). .. Single BFU-E or CFU-E cells were sorted to a 96-well plate and cultured with StemSpanTM SFEM medium (STEMCELL Technologies, Vancouver, BC, Canada) containing 100 ng/mL recombinant murine stem cell factor (rmSCF, STEMCELL Technologies, Vancouver, BC, Canada), 40 ng/mL recombinant murine insulin-like growth factor-1 (rmIGF-1, STEMCELL Technologies, Vancouver, BC, Canada), 2 U/mL recombinant human erythropoietin (rhEPO, Gibco, Billings, MT, USA), 100 U/mL penicillin and 100 μg/mL streptomycin and incubated at 37 °C in a humidified atmosphere (relative humidity > 80%) with 5% CO 2 . .. Sorted BM and FL erythroid progenitors were stained with CellTraceTM Violet dye (Thermo Fisher Scientific, Eugene, OR, USA) according to the instructions from the manufacturer and cultured in the medium as described above.

Incubation:

Article Title: Global Transcriptomic and Characteristics Comparisons between Mouse Fetal Liver and Bone Marrow Definitive Erythropoiesis.
Article Snippet: The cells were imaged using a Leica DM2000 inverted microscope (Leica Microsystems, Buffalo Grove, IL, USA). .. Single BFU-E or CFU-E cells were sorted to a 96-well plate and cultured with StemSpanTM SFEM medium (STEMCELL Technologies, Vancouver, BC, Canada) containing 100 ng/mL recombinant murine stem cell factor (rmSCF, STEMCELL Technologies, Vancouver, BC, Canada), 40 ng/mL recombinant murine insulin-like growth factor-1 (rmIGF-1, STEMCELL Technologies, Vancouver, BC, Canada), 2 U/mL recombinant human erythropoietin (rhEPO, Gibco, Billings, MT, USA), 100 U/mL penicillin and 100 μg/mL streptomycin and incubated at 37 ◦C in a humidified atmosphere (relative humidity > 80%) with 5% CO2. .. Sorted BM and FL erythroid progenitors were stained with CellTraceTM Violet dye (Thermo Fisher Scientific, Eugene, OR, USA) according to the instructions from the manufacturer and cultured in the medium as described above.

Article Title: Global Transcriptomic and Characteristics Comparisons between Mouse Fetal Liver and Bone Marrow Definitive Erythropoiesis
Article Snippet: The cells were imaged using a Leica DM2000 inverted microscope (Leica Microsystems, Buffalo Grove, IL, USA). .. Single BFU-E or CFU-E cells were sorted to a 96-well plate and cultured with StemSpanTM SFEM medium (STEMCELL Technologies, Vancouver, BC, Canada) containing 100 ng/mL recombinant murine stem cell factor (rmSCF, STEMCELL Technologies, Vancouver, BC, Canada), 40 ng/mL recombinant murine insulin-like growth factor-1 (rmIGF-1, STEMCELL Technologies, Vancouver, BC, Canada), 2 U/mL recombinant human erythropoietin (rhEPO, Gibco, Billings, MT, USA), 100 U/mL penicillin and 100 μg/mL streptomycin and incubated at 37 °C in a humidified atmosphere (relative humidity > 80%) with 5% CO 2 . .. Sorted BM and FL erythroid progenitors were stained with CellTraceTM Violet dye (Thermo Fisher Scientific, Eugene, OR, USA) according to the instructions from the manufacturer and cultured in the medium as described above.



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